MixtureAce™
First software capable of unleashing the power of massively parallel sequencing (MPS) for mixed casework, allowing users to more accurately analyze and interpret mixtures.
Industry experts have referred to massively parallel sequencing as a major breakthrough and the future of DNA analysis sequencing. As this sequencing approach becomes more commonly used in forensic labs, analysts need software that will allow them to adopt this technique.
MixtureAce™, developed by NicheVision, is the premier software for MPS. As a plug-in for ArmedXpert™, MixtureAce™ leverages the same user interface and mixture analysis functionality tools available through ArmedXpert™ – while allowing users to work with the MPS method.
Two of the primary functions of MixtureAce™ are:
- Analyzes MPS as easily as CE
- Automatically filters stutter and sequence artifacts, ultimately eliminating drop-in due to sequencer error and lower analytical thresholds for minor contributors
Overall, the intuitive features and functions of MixtureAce™ are ideal tools for ArmedXpert™ users to understand and transition to MPS data – and interpret more complex DNA data.
Features
- Simplifies the transition from capillary electrophoresis (CE)-based analysis to MPS analysis through ArmedXpert™
- Arranges MPS data through CE-equivalent formats, including allele tables and electropherogramic-like graphic displays
- Provides ArmedXpert™ mixture interpretation functions for MPS data
- Automatically identifies, labels and filters stutter and non-stutter artifacts, ultimately
- Files every allele and artifact “call” with “call” explanation through audit trail records
- Allows users to customize the extent of the polymerase chain reaction (PCR) amplicons analyzed from the Short Tandem Repeat (STR) locus proper to the entire amplicon sequence
- Labels sequence-based alleles and artifacts through the Sequence Identifier (SID) nomenclature, which can also be used a CE-based allele number nomenclature
- Provides users with the following tools: allele profile tables, interpretation tool, popout calls tool, hover over tool and more
Practical solutions. Proven expertise.
A complete pathway to operational, sequence-based STR analysis.
Operational MPS Requires More Than Instrumentation Alone.
Recent advances in forensic MPS chemistry, interpretation workflows, and implementation strategies are finally creating a practical pathway toward operational sequence-based STR analysis.
NicheVision provides the expertise, tools, and guidance to help laboratories successfully operationalize sequence-based STR analysis.
The Four Greatest Barriers to Operational MPS
Sequence-Based Interpretation
Complex mixture interpretation requires specialized algorithms and populatio ninformed models.NicheVision Solution →
NicheVision Solution
MixtureAce™ provides sequence-aware visualization and interpretation tools that help analysts evaluate isoalleles, artifacts, and complex DNA mixtures with confidence.
Evolution of Forensic Chemistry
Rapid chemistry innovation creates integration, standarization, and data consistency challenges.
NicheVision Solution →
NicheVision Solution
NicheVision provides expert guidance to help laboratories evaluate evolving chemistry options and select workflows that balance sensitivity, performance, and operational efficiency.
Analyst Adoption & Training
Effective adoption depends on intuitive tools, structured training, and analyst confidence.
NicheVision Solution →
NicheVision Solution
NicheVision provides structured training and onboarding to help analysts understand sequence-based data, build confidence, and transition successfully into operational MPS workflows.
Validation & Workflow Complexity
Robust validation frameworks and efficient workflows are essential for defensible, scalable operationsNicheVision Solution →
NicheVision Solution
NicheVision provides structured validation frameworks and implementation support to help laboratories develop reliable workflows, procedures, and competency standards for operational MPS.Who This Is For
Crime Laboratory Directors
Oversee strategic planning, resource allocation, and operational readiness.
Technical Leaders
Guide technology adoption, validation planning, and workflow integration.
Validation Teams
Design and execute robust studies that meet accreditation standards.
Forensic DNA Analysts
Apply and interpret complex data with confidence and consistency.
MixtureAce™
Purpose-Built for Sequence-Based
Forensic DNA Mixture Analysis
Powerful interpretation. Clear results. Defensible science.
Advanced probabilistic mixture interpretation
Activity level and contributor number assessments
Population-informed allele frequency models
Comprehensive reporting and audit trails
Intuitive, visual review environment
Seamless workflow integration
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